WB analysis using Immunochemicals Anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ?g loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kDa). The blot was incubated with a 1:500 dilution of the antibody at RT for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:1,000. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.