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ProductsBack/Neuropeptide S (NPS) Rabbit Polyclonal Antibody
WB Suggested Anti-NPS Antibody Titration: 1.0 ug/ml Positive Control: MCF7 Whole Cell
WB Suggested Anti-NPS Antibody Titration: 1.0 ug/ml Positive Control: MCF7 Whole Cell
WB Suggested Anti-NPS Antibody Titration: 1.0 ug/ml Positive Control: MCF7 Whole Cell

Neuropeptide S (NPS) Rabbit Polyclonal Antibody

TA358321
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetNPS
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Neuropeptide S (NPS) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID594857
  • Target name
    NPS
  • Target description
    neuropeptide S
  • Target synonyms
    neuropeptide S; prepro-neuropeptide S
  • Host
    Rabbit
  • Protein IDP0C0P6
  • Protein Name
    Neuropeptide S
  • Scientific Description
    NPS Antibody - N-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Neuropeptide S using anti-Neuropeptide S antibody (A01290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuropeptide S antigen affinity purified polyclonal antibody (Catalog # A01290-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuropeptide S at approximately 19KD. The expected band size for Neuropeptide S is at 10KD.
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