Western-Blot detection of human NRTN expressed in CHO cells. Lane 1: hNRTN-containing CHOEBNALT85 cell culture supernatant. Lane 2: Negative control - CHOEBNALT85 cell culturesupernatant. 5 microl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rb pAb to Chk IgY was used as secondary antibody. Antibody recognizes protein dimeric, and monomeric variants.