NF-kappaB Reporter Kit (NF-kappaB Signaling Pathway)

BPS Bioscience

NF-kappaB Reporter Kit (NF-kappaB Signaling Pathway)

7

Research Use Only

The NF-kappaB Reporter kit is designed for monitoring the activity of the NF-kappaB signaling pathway in the cultured cells. The kit contains transfection-ready NF-kappaB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-kappaB responsive element located upstream of a minimal promoter. The NF-kappaB reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

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