NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250. Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250. Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
NF-M antibody
GTX85461
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetNEFM
Overview
- SupplierGeneTex
- Product NameNF-M antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. IHC: 1:1000-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID4741
- Target nameNEFM
- Target descriptionneurofilament medium chain
- Target synonymsNEF3, NF-M, NFM, neurofilament medium polypeptide, 160 kDa neurofilament protein, neurofilament 3, neurofilament triplet M protein, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)
- HostChicken
- IsotypeIgY
- Protein IDP07197
- Protein NameNeurofilament medium polypeptide
- Scientific DescriptionNeurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Bruegger V et al., 2017 Jan 31, Nat CommunRead more
- HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. Bruegger V et al., 2015, PLoS BiolRead more
- Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon. Pelayo JC et al., 2014 May, Cell Tissue ResRead more
Datasheet
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