NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250. Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).

NF-M antibody

GTX85461

ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry

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Product group Antibodies

TargetNEFM

Overview

Supplier
GeneTex
Product Name
NF-M antibody
Delivery Days Customer
9
Application Supplier Note
WB: 1:1000-1:2000. IHC: 1:1000-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Concentration
2 mg/ml
Conjugate
Unconjugated
Gene ID4741
Target name
NEFM
Target description
neurofilament medium chain
Target synonyms
160 kDa neurofilament protein; NEF3; neurofilament 3; neurofilament medium polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NFM; NF-M
Host
Chicken
Isotype
IgY
Protein IDP07197
Protein Name
Neurofilament medium polypeptide
Scientific Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Storage Instruction
2°C to 8°C
UNSPSC
12352203

References

Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Bruegger V et al., 2017 Jan 31, Nat Commun
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HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. Bruegger V et al., 2015, PLoS Biol
Read more
Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon. Pelayo JC et al., 2014 May, Cell Tissue Res
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NF-M antibody

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Overview

References

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