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ProductsBack/NF2 Rabbit Polyclonal Antibody
WB Suggested Anti-NF2 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Brain
WB Suggested Anti-NF2 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Brain
WB Suggested Anti-NF2 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Brain

NF2 Rabbit Polyclonal Antibody

TA358369
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    NF2 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Scientific Description
    NF2 Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.
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