IHC-P analysis of human colon carcinoma tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of human spleen tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_1078_IHC-P_w_23060620_775.webp "IHC-P analysis of human spleen tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![IHC-P analysis of human tonsil tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_1079_IHC-P_w_23060620_146.webp "IHC-P analysis of human tonsil tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![ChIP analysis of 1x10? HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX22722 NFAT1 antibody [25A10.D6.D2]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene. IP reaction : 1.0ul antibody / 100ul sample](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_11_ChIP_w_23060620_548.webp "ChIP analysis of 1x10? HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX22722 NFAT1 antibody [25A10.D6.D2]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene. IP reaction : 1.0ul antibody / 100ul sample")
![ICC/IF analysis of MCF-7 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_373_ICC-IF_w_23060620_704.webp "ICC/IF analysis of MCF-7 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours using GTX22722 NFAT1 antibody [25A10.D6.D2]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_374_ICC-IF_w_23060620_760.webp "ICC/IF analysis of HeLa cells untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours using GTX22722 NFAT1 antibody [25A10.D6.D2]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature")
![ICC/IF analysis of HeLa cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_375_ICC-IF_w_23060620_731.webp "ICC/IF analysis of HeLa cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22722/GTX22722_376_ICC-IF_w_23060620_148.webp "ICC/IF analysis of U251 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
IHC-P analysis of human colon carcinoma tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
NFAT1 antibody [25A10.D6.D2]
GTX22722
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNfatc2
Overview
- SupplierGeneTex
- Product NameNFAT1 antibody [25A10.D6.D2] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross react with NFAT2 (NFATc, NFATc1).
- Application Supplier NoteWB: 1 microg/ml. ICC/IF: 1:100 - 1:1000. IHC-P: 1:50-1:200. IHC-Fr: 1:50-1:200. ChIP assay: 1-3 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID25A10.D6.D2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID18019
- Target nameNfatc2
- Target descriptionnuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
- Target synonymsAI607462; NF; NFA; NFAT pre-existing subunit; NFAT1; NFAT1-D; NF-ATc2; Nfatp; NF-ATp; nuclear factor of activated T-cells, cytoplasmic 2; T-cell transcription factor NFAT1
- HostMouse
- IsotypeIgG1
- Protein IDQ60591
- Protein NameNuclear factor of activated T-cells, cytoplasmic 2
- Scientific DescriptionThe nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- CD5 promotes IL-10 production in chronic lymphocytic leukemia B cells through STAT3 and NFAT2 activation. Garaud S et al., 2011 Apr 15, J ImmunolRead more