WB analysis of whole cell extracts (30 microg lysate) of NIH/3T3 (Lane 1) treated for 20 minutes with 200 nM of PMA (Lane 2), Jurkat (Lane 3) treated for 20 minutes with 200 nM of PMA (Lane 4) using GTX25246 NFAT1 (phospho Ser54) antibody. Dilution : 1:500-1:2000
WB analysis of whole cell extracts (30 microg lysate) of NIH/3T3 (Lane 1) treated for 20 minutes with 200 nM of PMA (Lane 2), Jurkat (Lane 3) treated for 20 minutes with 200 nM of PMA (Lane 4) using GTX25246 NFAT1 (phospho Ser54) antibody. Dilution : 1:500-1:2000
NFAT1 (phospho Ser54) antibody
GTX25246
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNfatc2
Overview
- SupplierGeneTex
- Product NameNFAT1 (phospho Ser54) antibody
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID18019
- Target nameNfatc2
- Target descriptionnuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
- Target synonymsNF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp, nuclear factor of activated T-cells, cytoplasmic 2, NFAT pre-existing subunit, T-cell transcription factor NFAT1
- HostRabbit
- IsotypeIgG
- Protein IDQ60591
- Protein NameNuclear factor of activated T-cells, cytoplasmic 2
- Scientific DescriptionNuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT 1 (also known as NFATc2 or NFATp) is a 154 kDa member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT 1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT 1 has been shown to be important in the regulation of its transcriptional activity.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Banerjee A, Li D, Guo Y, et al. A reengineered common chain cytokine augments CD8+ T cell-dependent immunotherapy. JCI Insight. 2022,7(10). doi: 10.1172/jci.insight.158889Read this paper
- So JS, Kim GC, Song M, et al. 6-Methoxyflavone inhibits NFAT translocation into the nucleus and suppresses T cell activation. J Immunol. 2014,193(6):2772-83. doi: 10.4049/jimmunol.1400285Read this paper
Datasheet
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