Bio-Connect

NFkB p65 antibody

GTX102090
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRELA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    NFkB p65 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.49 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5970
  • Target name
    RELA
  • Target description
    RELA proto-oncogene, NF-kB subunit
  • Target synonyms
    CMCU; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04206
  • Protein Name
    Transcription factor p65
  • Scientific Description
    NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • 15-keto-PGE(2) alleviates nonalcoholic steatohepatitis through its covalent modification of NF-kappaB factors.
    Read more
  • Antioxidant and anti-inflammatory effects of allicin in the kidney of an experimental model of metabolic syndrome.
    Read more
  • Gastroprotective effects of Machilus zuihoensis Hayata bark against acidic ethanol-induced gastric ulcer in mice.
    Read more
  • DNA-dependent protein kinase catalytic subunit binds to the transactivation domain 1 of NF-kappaB p65.
    Read more
  • Calcineurin B1 Deficiency Reduces Proliferation, Increases Apoptosis, and Alters Secretion in Enteric Glial Cells of Mouse Small Intestine in Culture.
    Read more
  • Electroacupuncture relieves chronic pain by promoting microglia M2 polarization in lumbar disc herniation rats.
    Read more
  • ANGPTL2 promotes VEGF-A synthesis in human lung cancer and facilitates lymphangiogenesis.
    Read more
  • Cisplatin or Doxorubicin Reduces Cell Viability via the PTPIVA3-JAK2-STAT3 Cascade in Hepatocellular Carcinoma.
    Read more
  • Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
    Read more
  • Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-kappaB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
    Read more

Related products

Product group Antibodies
Assay Genie
TargetRELA
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetRELA
  • SizePrice
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetRELA
  • SizePrice
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Product group Antibodies
References
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRELA
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
TargetRELA
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRELA
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRELA
  • SizePrice
WB analysis of Jurkat (1), K562 (2) and NIH3T3 (3) cell lysate using GTX83260 NFkB p65 antibody [6H7].
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
TargetRELA
  • SizePrice
IHC-P analysis of human ovarian cystadenocarcinoma tissue using GTX13594 NFkB p65 antibody [112A1021]. Dilution : 5 microg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, Mass Spectrometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRELA
  • SizePrice
IHC-P analysis of mouse spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody. Right : Primary antibody Left : a negative control without primary antibody Dilution : 1:50 overnight at 4oC Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRELA
  • SizePrice