NGF 2.5S antibody

GeneTex

NGF 2.5S antibody

9

May be used in monitoring for NGF (2.5S, β and 7S).

WB: 1:2,000. Dot: 1:2,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Dot Blot, ImmunoDiffusion, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, Neutralisation/Blocking

Research Use Only

Polyclonal

Unconjugated

Ngf

nerve growth factor

beta-nerve growth factor; beta-NGF; nerve growth factor, beta; Ngfb

Rabbit

IgG

Beta-nerve growth factor

NGF is purified in three forms: the 7S, 2.5S and b. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two a, one b and two g subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the b, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the b form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.

Human, Mouse

-20°C or -80°C,2°C to 8°C

12352203