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NHEJ1 / XLF Antibody (clone OTI1F3)

LS-C172445
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNHEJ1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NHEJ1 / XLF Antibody (clone OTI1F3)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:100), IHC, IHC-P (1:150), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1F3
  • Concentration
    0.77 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79840
  • Target name
    NHEJ1
  • Target description
    non-homologous end joining factor 1
  • Target synonyms
    non-homologous end-joining factor 1; nonhomologous end-joining factor 1; protein cernunnos; XLF; XRCC4-like factor
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHEJ1 antigen affinity purified polyclonal antibody (Catalog # A03552-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHEJ1 at approximately 38KD. The expected band size for NHEJ1 is at 33KD.
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