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ProductsBack/NKCC1 (SLC12A2) Rabbit Polyclonal Antibody
Western blot analysis of extracts of mouse pancreas, using SLC12A2 antibody (TA381540) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 90s.
Western blot analysis of extracts of mouse pancreas, using SLC12A2 antibody (TA381540) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 90s.
Western blot analysis of extracts of mouse pancreas, using SLC12A2 antibody (TA381540) at 1:3000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 90s.

NKCC1 (SLC12A2) Rabbit Polyclonal Antibody

TA381540
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSLC12A2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    NKCC1 (SLC12A2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6558
  • Target name
    SLC12A2
  • Target description
    solute carrier family 12 member 2
  • Target synonyms
    BSC, BSC-2, BSC2, CCC1, KILQS, NKCC1, PPP1R141, hNKCC1, solute carrier family 12 member 2, Na-K-2Cl cotransporter 1, basolateral Na-K-Cl symporter, bumetanide-sensitive cotransporter type 2, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, protein phosphatase 1, regulatory subunit 141, solute carrier family 12 (sodium/potassium/chloride transporter), member 2, solute carrier family 12 (sodium/potassium/chloride transporters), member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55011
  • Protein Name
    Solute carrier family 12 member 2
  • Scientific Description
    SLC12A2 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (A03603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKCC1/SLC12A2 antigen affinity purified polyclonal antibody (Catalog # A03603) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NKCC1/SLC12A2 at approximately 180 kDa. The expected band size for NKCC1/SLC12A2 is at 180 kDa.
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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
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