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ProductsBack/NLE1 Antibody (aa351-400, HRP)

NLE1 Antibody (aa351-400, HRP)

LS-C473042
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNLE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NLE1 Antibody (aa351-400, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.65 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID54475
  • Target name
    NLE1
  • Target description
    notchless homolog 1
  • Target synonyms
    NLE; Notchless gene homolog; notchless protein homolog 1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NLE1 using anti-NLE1 antibody (A09529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLE1 antigen affinity purified polyclonal antibody (A09529-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLE1 at approximately 45 kDa. The expected band size for NLE1 is at 53 kDa.
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