nNOS antibody

GeneTex

nNOS antibody

9

This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).

WB: 1:1000. ICC/IF: 1:250. IHC-P: 1:200-400. IHC-Fr: 1:800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

Nos1

nitric oxide synthase 1, neuronal

2310005C01Rik; b; bNOS; constitutive NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxide synthase, brain; nNOS; N-NOS; NO; NOS; NOS type I; Nos-1; NOS-I; peptidyl-cysteine S-nitrosylase NOS1

Rabbit

IgG

Nitric oxide synthase, brain

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells.

Bovine, Guinea Pig, Human, Mouse, Rabbit, Rat, Zebra Fish

-20°C or -80°C,2°C to 8°C

12352203