NPY1R Antibody (aa180-229) LS-B3546

Biorbyt

NPY1R Antibody (aa180-229) LS-B3546

10

Further information: Immunohistochemistry: LS-B3546 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3546 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

IHC, IHC-P (5 µg/ml), WB IHC, IHC-P, WB

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

Rabbit

IgG

NPY1R Antibody (aa180-229) LS-B3546

Human, Mouse

-20°C,2°C to 8°C

12352203