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ProductsBack/Nucleophosmin (phospho Thr234) antibody
WB analysis of colchicine-treated MCF7 (A), LNCaP (B), HeLa (C), colchicine-treated Raw264.7 (D), colchicine-treated PC12 (E) whole cell lysates using GTX32229 Nucleophosmin (phospho Thr234) antibody.
WB analysis of colchicine-treated MCF7 (A), LNCaP (B), HeLa (C), colchicine-treated Raw264.7 (D), colchicine-treated PC12 (E) whole cell lysates using GTX32229 Nucleophosmin (phospho Thr234) antibody.
WB analysis of colchicine-treated MCF7 (A), LNCaP (B), HeLa (C), colchicine-treated Raw264.7 (D), colchicine-treated PC12 (E) whole cell lysates using GTX32229 Nucleophosmin (phospho Thr234) antibody.

Nucleophosmin (phospho Thr234) antibody

GTX32229
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetNPM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Nucleophosmin (phospho Thr234) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4869
  • Target name
    NPM1
  • Target description
    nucleophosmin 1
  • Target synonyms
    B23; NPM; nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; testicular tissue protein Li 128
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06748
  • Protein Name
    Nucleophosmin
  • Scientific Description
    The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). Nucleophosmin was detected in a paraffin-embedded section of Mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Nucleophosmin Antibody (PB9341) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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