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NUP54 Antibody (aa302-351)

LS-C169797
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNUP54
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUP54 Antibody (aa302-351)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID53371
  • Target name
    NUP54
  • Target description
    nucleoporin 54
  • Target synonyms
    54 kDa nucleoporin; nucleoporin 54kD; nucleoporin 54kDa; nucleoporin p54
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP54 using anti-NUP54 antibody (A10830-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP54 antigen affinity purified polyclonal antibody (Catalog # A10830-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP54 at approximately 56 kDa. The expected band size for NUP54 is at 56 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
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