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OIT3 Antibody (aa297-506)

LS-C295718
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetOIT3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    OIT3 Antibody (aa297-506)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (5 - 10 µg/ml), WB (0.5 - 2 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID170392
  • Target name
    OIT3
  • Target description
    oncoprotein induced transcript 3
  • Target synonyms
    liver-specific zona pellucida domain-containing protein; liver-specific ZP domain-containing protein; LZP; oncoprotein-induced transcript 3 protein
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OIT3 using anti-OIT3 antibody (A12768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue(HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIT3 antigen affinity purified polyclonal antibody (Catalog # A12768-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIT3 at approximately 51 kDa. The expected band size for OIT3 is at 60 kDa.
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