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ProductsBack/OPG antibody [bony-1]
FACS analysis of cells with MAb to OPG (human) (GTX18051). HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50microl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1microg/ml of MAb to OPG (human) (Bony-1) (GTX18051). After washing in FACS buffer, RPE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
FACS analysis of cells with MAb to OPG (human) (GTX18051). HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50microl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1microg/ml of MAb to OPG (human) (Bony-1) (GTX18051). After washing in FACS buffer, RPE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
FACS analysis of cells with MAb to OPG (human) (GTX18051). HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50microl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1microg/ml of MAb to OPG (human) (Bony-1) (GTX18051). After washing in FACS buffer, RPE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

OPG antibody [bony-1]

Research Use Only
GTX18051
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation
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Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    GeneTex
  • Product Name
    OPG antibody [bony-1]
  • Delivery Days Customer
    9
  • Antibody Specificity
    Recognizes human OPG
  • Applications
    Flow Cytometry, ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    bony-1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C,-20°C or -80°C
  • UNSPSC
    12352203