Other 1,5-AG ELISA Kit
ORB781677
ReactivityAll Species
Product group Assays
Overview
- SupplierBiorbyt
- Product NameOther 1,5-AG ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 150 microg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve
- ApplicationsELISA
- Applications SupplierELISA
- Assay Detection Range2.35-150 microg/mL
- Assay Sensitivity0.6 microg/mL
- CertificationResearch Use Only
- Scientific DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-Anhydroglucitol(1,5-AG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-Anhydroglucitol(1,5-AG).
- ReactivityAll Species
- UNSPSC41116158