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Overexpressing lysates

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DNA-binding activity of EGR2 was measured in OriGene over-expression lysate LY424742 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing EGR2-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed EGR2 is biologically active in the lysate. Overexpression cell lysates are prepared from HEK293T cells transfected with RC211904 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
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Western blot validation of overexpression lysate (Cat# LY414189) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212483 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
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Western blot validation of overexpression lysate (Cat# LY424151) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214437 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
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Western blot validation of overexpression lysate (Cat# LY424152) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220328 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424154) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218574 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424157) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212366 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424160) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214068 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424163) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222056 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424165) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211371 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY419311) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213170 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424172) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207344 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY424173) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216895 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
  • SizePrice