oYo-Link Thiol Antibody Labeling Reagent - Labels 1mg

AlphaThera

oYo-Link Thiol Antibody Labeling Reagent - Labels 1mg

9

Thiol-Maleimide Coupling reactions; Reactions with Thiol-reactive Groups

Research Use Only

270 ug/mL

Liquid

Research Use Only Applications: Reactions with Thiol-reactive Groups oYo-Link Thiol enables the site-specific labeling of oYo-Link-compatible antibodies with a Thiol to the IgG heavy chain. oYo-Link Thiol limits the attachment of the thiol labels to the heavy chains of the antibody and therefore ensures that the thiol label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 Thiol labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link Thiol with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins. (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaThera's LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support@alphathera.com

4°C for short-term durations (< 1 week) and -20°C to -80°C for longer time-spans

12352203