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p120 Monoclonal Antibody

RD6752
Reddot Biotech
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCTNND1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    p120 Monoclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Concentration
    100 ug/ml
  • Gene ID1500
  • Target name
    CTNND1
  • Target description
    catenin delta 1
  • Target synonyms
    BCDS2, CAS, CTNND, P120CAS, P120CTN, p120, p120(CAS), p120(CTN), catenin delta-1, cadherin-associated Src substrate, catenin (cadherin-associated protein), delta 1, p120 catenin
  • Host
    Mouse
  • Scientific Description
    The linker family includes Oe+/- - Catenin, beta - Catenin, r-catenin and p120 catenin. P120 catenin is linked to E-cadherin in the cytoplasm near the cell membrane, forming a complex, which is stable and closely linked. If E-cadherin is absent, p120 catenin will accumulate in the cytoplasm. There is only a small amount of membrane expression in breast ductal carcinoma, but there is no p120 catenin accumulation in the cytoplasm. The antibody can be used to differentiate breast lobular / ductal tumors.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of delta 1 Catenin/CAS/CTNND1 using anti-delta 1 Catenin/CAS/CTNND1 antibody (A02333-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: rat RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-delta 1 Catenin/CAS/CTNND1 antigen affinity purified polyclonal antibody (Catalog # A02333-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for delta 1 Catenin/CAS/CTNND1 at approximately 100-110 kDa. The expected band size for delta 1 Catenin/CAS/CTNND1 is at 108 kDa.
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