Western blot analysis of extracts of K562 cells, using Phospho-CRK/CRKII-Y221 antibody (TA375098) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour . - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 10s.
Western blot analysis of extracts of K562 cells, using Phospho-CRK/CRKII-Y221 antibody (TA375098) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour . - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 10s.
p38 (CRK) Rabbit Polyclonal Antibody
TA375098
Overview
- SupplierOriGene
- Product Namep38 (CRK) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID1398
- Target nameCRK
- Target descriptionCRK proto-oncogene, adaptor protein
- Target synonymsadapter molecule crk; CRKII; p38; proto-oncogene c-Crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene-like protein
- HostRabbit
- IsotypeIgG
- Protein IDP46108
- Protein NameAdapter molecule crk
- Scientific DescriptionPhospho-CRK/CRKII-Y221 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203