Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 antibody (TA378307) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 antibody (TA378307) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
p38 (MAPK14) Rabbit Polyclonal Antibody
TA378307
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK11
Overview
- SupplierOriGene
- Product Namep38 (MAPK14) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5600
- Target nameMAPK11
- Target descriptionmitogen-activated protein kinase 11
- Target synonymsMAP kinase 11; MAP kinase p38 beta; mitogen-activated protein kinase 11; mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; p38-2; P38B; p38Beta; P38BETA2; PRKM11; SAPK2; SAPK2B; stress-activated protein kinase-2; stress-activated protein kinase-2b
- HostRabbit
- IsotypeIgG
- Protein IDQ15759
- Protein NameMitogen-activated protein kinase 11
- Scientific DescriptionPhospho-p38 MAPK-T180/Y182 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203