p53 Antibody / TP53

Biorbyt

p53 Antibody / TP53

16

Optimal dilution of the antibody should be determined by the researcher.

ELISA

ELISA (order BSA-free format for coating) ELISA

Research Use Only

Monoclonal

PAb240

Unconjugated

TP53

tumor protein p53

antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53

Mouse

IgG1

Cellular tumor antigen p53

The specificity of this monoclonal antibody to its intended target was tested by HuProtTM Array, containing more than 19,000, full-length human proteins. PAb240 binds to the C-terminus (aa213-217) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Human

-20°C,2°C to 8°C

12352203