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MCF-7 cells were treated with 50 uM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells.
MCF-7 cells were treated with 50 uM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells.
MCF-7 cells were treated with 50 uM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells.

p53 (TP53) Rabbit Polyclonal Antibody [Clone ID: Poly6142]

Research Use Only
TA327919
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTP53
200 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    p53 (TP53) Rabbit Polyclonal Antibody [Clone ID: Poly6142]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Poly6142
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit Polyclonal anti-p53-Acetylated (Lys382) Antibody
  • Reactivity
    Human
  • UNSPSC
    12352203