Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (TA382723) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (TA382723) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
p53 (TP53) Rabbit Polyclonal Antibody
TA382723
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTP53
Overview
- SupplierOriGene
- Product Namep53 (TP53) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID7157
- Target nameTP53
- Target descriptiontumor protein p53
- Target synonymsantigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-p53-S33 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- UNSPSC12352203