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Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (TA382723) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (TA382723) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (TA382723) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.

p53 (TP53) Rabbit Polyclonal Antibody

Research Use Only
TA382723
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTP53
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    p53 (TP53) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7157
  • Target name
    TP53
  • Target description
    tumor protein p53
  • Target synonyms
    antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04637
  • Protein Name
    Cellular tumor antigen p53
  • Scientific Description
    Phospho-p53-S33 Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203