Western blot analysis of extracts of various cell lines, using Phospho-p53-S37 antibody (TA382728) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Phospho-p53-S37 antibody (TA382728) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 1s.
p53 (TP53) Rabbit Polyclonal Antibody
TA382728
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTP53
Overview
- SupplierOriGene
- Product Namep53 (TP53) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID7157
- Target nameTP53
- Target descriptiontumor protein p53
- Target synonymsantigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-p53-S37 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- UNSPSC12352203