PathPlus(tm) Collagen IV Antibody
LS-B8976
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Porcine, Rat
Overview
- SupplierLifeSpan BioSciences
- Product NamePathPlus(tm) Collagen IV Antibody
- Delivery Days Customer23
- Antibody SpecificityRecognizes extracellular - basement membranes.
- Application Supplier NoteMethanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.. ICC, IHC, IHC-Fr (1:25 - 1:100), IHC-P (1:75 - 1:100), WB (1:100 - 1:1000) Methanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.
- ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Human, Porcine, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203