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PathPlus(tm) Collagen IV Antibody

Research Use Only
LS-B8976
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Porcine, Rat
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) Collagen IV Antibody
  • Delivery Days Customer
    23
  • Antibody Specificity
    Recognizes extracellular - basement membranes.
  • Application Supplier Note
    Methanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.. ICC, IHC, IHC-Fr (1:25 - 1:100), IHC-P (1:75 - 1:100), WB (1:100 - 1:1000) Methanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Human, Porcine, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203