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PathPlus(tm) MRE11A / MRE11 Antibody

Research Use Only
LS-B1447
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetMRE11
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) MRE11A / MRE11 Antibody
  • Delivery Days Customer
    23
  • Antibody Specificity
    A BLAST analysis was used to suggest cross reactivity with Mre11 protein from rat (94% homology). Based on protein sequence homology also expect partial reactivity against Mre11 homologues from macaque (88%), human (84%) and dog (82%). This sequence shows
  • Application Supplier Note
    Immunohistochemistry: LS-B1447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1447 was determined to be 5 ug/ml.. ELISA (1:10000 - 1:50000), IHC-P (2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B1447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1447 was determined to be 5 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4361
  • Target name
    MRE11
  • Target description
    MRE11 homolog, double strand break repair nuclease
  • Target synonyms
    ATLD; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11; double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11-like protein A; MRE11A; MRE11B
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    12352203