IHC-P analysis of human kidney tissue using GTX31119 PAX8 antibody [1F8-3A8]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
![IHC-P analysis of human thyroid tissue using GTX31119 PAX8 antibody [1F8-3A8]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX31119/GTX31119_1336_IHC-P_w_23060722_345.webp "IHC-P analysis of human thyroid tissue using GTX31119 PAX8 antibody [1F8-3A8]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![IHC-P analysis of mouse kidney tissue using GTX31119 PAX8 antibody [1F8-3A8]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX31119/GTX31119_1337_IHC-P_w_23060722_388.webp "IHC-P analysis of mouse kidney tissue using GTX31119 PAX8 antibody [1F8-3A8]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20")
![WB analysis of 20 ug of the indicated whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX31119/GTX31119_1845_WB_w_23060722_224.webp "WB analysis of 20 ug of the indicated whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8]. Dilution : 1:500")
![WB analysis of 60 ug of SKOV-3, COS7, and negative control HeLa whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31119/GTX31119_1846_WB_w_23060722_298.webp "WB analysis of 60 ug of SKOV-3, COS7, and negative control HeLa whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8]. Dilution : 1:1000")
![WB analysis of 25 ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane) using GTX31119 PAX8 antibody [1F8-3A8]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31119/GTX31119_2082_WB_w_23060722_876.webp "WB analysis of 25 ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane) using GTX31119 PAX8 antibody [1F8-3A8]. Dilution : 1:1000")
![ICC/IF analysis of 293T (left panel) and COS7 cells (right panel) using GTX31119 PAX8 antibody [1F8-3A8]. Green : PAX8 Red: F-actin Blue: Hoechst 33342 Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX31119/GTX31119_732_ICC-IF_w_23060722_167.webp "ICC/IF analysis of 293T (left panel) and COS7 cells (right panel) using GTX31119 PAX8 antibody [1F8-3A8]. Green : PAX8 Red: F-actin Blue: Hoechst 33342 Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
IHC-P analysis of human kidney tissue using GTX31119 PAX8 antibody [1F8-3A8]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
PAX8 antibody [1F8-3A8]
GTX31119
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate
TargetPAX8
Overview
- SupplierGeneTex
- Product NamePAX8 antibody [1F8-3A8] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:20-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1F8-3A8
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7849
- Target namePAX8
- Target descriptionpaired box 8
- Target synonymspaired box protein Pax-8; paired domain gene 8
- HostMouse
- IsotypeIgG1
- Protein IDQ06710
- Protein NamePaired box protein Pax-8
- Scientific DescriptionThis gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
- ReactivityHuman, Mouse, Primate
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203