PC2 antibody

Biorbyt

PC2 antibody

16

Anti-PC2 antibody has been tested for use in ELISA, WB, and IF. This affinity purified antibody is useful in immunofluorescence staining of cultured cells. In immunofluorescence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. This affinity purified antibody is useful in western blotting using transfected cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein. Reactivity in other immunoassays is unknown.

ELISA: 1:10,000 - 1:40,000, IF: 1:100 - 1:400, IP: 1:100, WB: 1:1,000 - 1:4,000 ELISA, IF, IP, WB

Research Use Only

Polyclonal

1.36 mg/ml

Unconjugated

Liquid

CBX4

chromobox 4

chromobox homolog 4 (Pc class homolog, Drosophila); chromobox protein homolog 4; chromobox-like protein 4; E3 SUMO-protein ligase CBX4; NBP16; NS5ATP1-binding protein 16; Pc class 2 homolog; PC2; polycomb 2 homolog

Rabbit

IgG

E3 SUMO-protein ligase CBX4

PC2 antibody

Human

-20°C,2°C to 8°C

12352203