WB using Anti-Alga PCNA antibody shows detection of a predominant band at ~29 kDa corresponding to PCNA (arrowhead lane 2). Peptide competition effectively eliminates specific band reactivity (lane 1). For peptide competition, the antibody was pre-incubated with 100 ug (excess) of the peptide for 2h at 37°C followed by overnight incubation at 4°C with 100 ug (excess) of the peptid. Primary antibody diluted to 1:2001000.