PDE10A2 Assay Kit

BPS Bioscience

PDE10A2 Assay Kit

7

1 x 96 wells

Research Use Only

The PDE10A2 Assay Kit is a fluorescence polarization (FP), homogeneous, 96-well assay kit designed for the screening and profiling of PDE10A2 (Phosphodiesterase 10A2) inhibitors. This assay takes advantage of a specific fluorescent phosphate-binding nanoparticle. The kit contains enough purified recombinant PDE10A2, fluorescent probe, PDE assay buffer, Binding Agent, and diluent for 100 reactions. The assay uses a fluorescein-labeled cyclic adenosine monophosphate (cAMP-FAM for PDE10A2), in which the phosphate group is engaged within the cyclic nucleotide. This is a very small molecule that rotates fast (low FP). PDE10A2 catalyzes the hydrolysis of the phosphodiester bond in the cyclic nucleotide and frees the phosphate group. In a second step the free phosphate group is recognized by a specific phosphate-binding nanobead (Binding Agent) leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to PDE10A2 activity.

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