PDE1B-HEK293 Recombinant Cell line

BPS Bioscience

PDE1B-HEK293 Recombinant Cell line

7

Research Use Only

PDE1B

phosphodiesterase 1B

HEL-S-79p, PDE1B1, PDES1B, dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, 63 kDa Cam-PDE, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase, calmodulin-stimulated phosphodiesterase PDE1B1, cam-PDE 1B, epididymis secretory sperm binding protein Li 79p, phosphodiesterase 1B, calmodulin-dependent, presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924). N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca2+ influx through CNGA2, acting as the intracellular cGMP sensor.

Liquid Nitrogen

41116106

Human