PDI antibody [1D3]

GeneTex

PDI antibody [1D3]

9

For ICC: Use at a concentration of 2-5 microg/ml. For IP: Use at a concentration of 8-12 microg/ml. For WB: Use at a concentration of 2-4 microg/ml. Predicted molecular weight: 58 kDa. Optimal dilutions/concentrations should be determined by the researcher.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

1D3

1 mg/ml

Unconjugated

P4hb

prolyl 4-hydroxylase subunit beta

cellular thyroid hormone-binding protein; PDI; PDIR; prolyl 4-hydroxylase, beta polypeptide; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide-isomerase

Mouse

IgG1

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Bovine, Canine, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep, Xenopus

-20°C or -80°C,2°C to 8°C

12352203