![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104286/GTX104286_42760_20180810_WB_B_w_23060120_814.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PDK3 antibody [N1C2]
GTX104286
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Human, Mammals, Mouse
TargetPDK3
Overview
- SupplierGeneTex
- Product NamePDK3 antibody [N1C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.16 mg/ml
- ConjugateUnconjugated
- Gene ID5165
- Target namePDK3
- Target descriptionpyruvate dehydrogenase kinase 3
- Target synonymsCMTX6, GS1-358P8.4, pyruvate dehydrogenase kinase, isozyme 3, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, pyruvate dehydrogenase kinase, isoenzyme 3, pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial
- HostRabbit
- IsotypeIgG
- Protein IDQ15120
- Protein Name[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
- Scientific DescriptionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
- ReactivityAmphibian, Human, Mammals, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Jonus HC, Byrnes CC, Kim J, et al. Thiamine mimetics sulbutiamine and benfotiamine as a nutraceutical approach to anticancer therapy. Biomed Pharmacother. 2020,121:109648. doi: 10.1016/j.biopha.2019.109648Read this paper
- Al-Attar R, Wijenayake S, Storey KB. Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia. Cryobiology. 2019,86:10-18. doi: 10.1016/j.cryobiol.2019.01.006Read this paper


![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104286/GTX104286_42760_20170309_WB_w_23060120_345.webp)
![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104286/GTX104286_40002_20170119_WB_M_liver_w_23060120_747.webp)





