Goat anti Beta phosphoglucomutase antibody was used to detect purified Beta phosphoglucomutase under reducing (R) and non-reducing (NR) conditions. Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1/3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated donkey anti goat 1/10K in TBS/blocking buffer 1 hr RT). Images were collected using the BioRad VersaDoc System.