More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PHB / Prohibitin Antibody

PHB / Prohibitin Antibody

LS-C812751
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPHB
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PHB / Prohibitin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5245
  • Target name
    PHB
  • Target description
    prohibitin
  • Target synonyms
    epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; HEL-215; HEL-S-54e; PHB1; prohibitin
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-PHB Antibody144-00056
ApplicationsWestern Blot
TargetPHB
  • SizePrice
Product group Antibodies
Biosynth
PHB antibody70R-19252
  • SizePrice
Product group Antibodies
Biomatik
PHB Polyclonal AntibodyCAC13157
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetPHB
  • SizePrice
Product group Antibodies
Assay Genie
Prohibitin AntibodyCAB0056
TargetPHB
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA014587(PHB Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
Product group Antibodies
Cusabio
PHB AntibodyCSB-PA014587
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPHB
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PHB / Prohibitin AntibodyLS-C400968
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPHB
  • SizePrice
Anti-PHB Antibody
Product group Antibodies
Antibodies.com
Anti-PHB AntibodyA29414
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Prohibitin Antibody, Affinity PurifiedA302-014A
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetPHB
  • SizePrice
Mouse heart lysates probed with Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss Antibodies
Prohibitin Polyclonal AntibodyBS-1449R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPHB
  • SizePrice
Figure 1. Western blot analysis of Prohibitin/PHB using anti-Prohibitin/PHB antibody (A01178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human Sw620 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: human A549 whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat PC-12 whole cell lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prohibitin/PHB antigen affinity purified polyclonal antibody (Catalog # A01178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prohibitin/PHB at approximately 28-30KD. The expected band size for Prohibitin/PHB is at 28-30KD.
Product group Antibodies
Boster Bio
Anti-Prohibitin/PHB Antibody Picoband(r)A01178-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPHB
  • SizePrice