Phi29 DNA Polymerase

Applied Biological Materials

Phi29 DNA Polymerase

9

Research Use Only

Ultra-Pure Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Ultra-Pure Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3 to 5 exonuclease activity and can amplify from very small amounts of starting templates. The enzyme is subject to a rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA. Quality control involves a non-specific DNase Activity Assay and qPCR DNA Contamination Test with a TaqMan probe.

-20°C

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