Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-ATF2(T71)) Monoclonal Antibody, Unconjugated (bsm-54546R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-ATF2(T71)) Monoclonal Antibody, Unconjugated (bsm-54546R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Phospho-ATF2(T71) Monoclonal Antibody
BSM-54546R
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetATF2
Overview
- SupplierBioss Antibodies
- Product NamePhospho-ATF2(T71) Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- Applications SupplierWB(1:300-5000), IF(ICC)(1:50-200), IP(1-2ug)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10F3
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1386
- Target nameATF2
- Target descriptionactivating transcription factor 2
- Target synonymsactivating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB2; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; TREB7
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDP15336
- Protein NameCyclic AMP-dependent transcription factor ATF-2
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203