Immunofluorescence staining of A549 cells with CSB-RA618017A255phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Phospho-SMAD2 (S255) Recombinant Monoclonal Antibody
CSB-RA618017A255PHHU
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetSMAD2
Overview
- SupplierCusabio
- Product NamePhospho-SMAD2 (S255) Recombinant Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A12
- ConjugateUnconjugated
- Gene ID4087
- Target nameSMAD2
- Target descriptionSMAD family member 2
- Target synonymshMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; MADH2; MADR2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2
- IsotypeIgG
- Protein IDQ15796
- Protein NameMothers against decapentaplegic homolog 2
- Scientific DescriptionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203