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Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFbeta RI (S165) Polyclonal Antibody
Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFbeta RI (S165) Polyclonal Antibody
Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFbeta RI (S165) Polyclonal Antibody

Phospho-TGFBR1 (S165) Antibody

CSB-PA050248
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTGFBR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-TGFBR1 (S165) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7046
  • Target name
    TGFBR1
  • Target description
    transforming growth factor beta receptor 1
  • Target synonyms
    AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I, TGF-beta receptor type-1, activin A receptor type II-like kinase, 53kDa, activin A receptor type II-like protein kinase of 53kD, activin receptor-like kinase 5, mutant transforming growth factor beta receptor I, serine/threonine-protein kinase receptor R4, transforming growth factor beta receptor I, transforming growth factor-beta receptor type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36897
  • Protein Name
    TGF-beta receptor type-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGF Beta Receptor I/TGFBR1 using anti-TGF Beta Receptor I/TGFBR1 antibody (A00832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor I/TGFBR1 antigen affinity purified polyclonal antibody (Catalog # A00832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor I/TGFBR1 at approximately 56 kDa. The expected band size for TGF Beta Receptor I/TGFBR1 is at 56 kDa.
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