ELISA results of Mab anti-phosphothreonine antibody (GTX22286) tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1microg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) antibody and TMB substrate were used for detection.
![Mab anti-phosphothreonine antibody (clone 18F6, GTX22286) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1,000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using 1:2,500 dilution Goat-a-Mouse IgG [H&L] secondary antibody and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.](https://www.genetex.com/upload/website/prouct_img/normal/GTX22286/GTX22286_20160330_WB_w_23060620_444.webp "Mab anti-phosphothreonine antibody (clone 18F6, GTX22286) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1,000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using 1:2,500 dilution Goat-a-Mouse IgG [H&L] secondary antibody and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.")
![ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22286 Phosphothreonine antibody [18F6]. Coating : 0.1 microg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22286/GTX22286_20240423_ELISA_37_24042320_518.webp "ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22286 Phosphothreonine antibody [18F6]. Coating : 0.1 microg Dilution : 1:1000")
![WB analysis of EGF-treated A431 whole cell lysate using GTX22286 Phosphothreonine antibody [18F6]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22286/GTX22286_20240423_WB_38_24042320_320.webp "WB analysis of EGF-treated A431 whole cell lysate using GTX22286 Phosphothreonine antibody [18F6]. Dilution : 1:1000")
ELISA results of Mab anti-phosphothreonine antibody (GTX22286) tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1microg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) antibody and TMB substrate were used for detection.
Phosphothreonine antibody [18F6]
GTX22286
Overview
- SupplierGeneTex
- Product NamePhosphothreonine antibody [18F6]
- Delivery Days Customer9
- Antibody SpecificityReactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:4000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID18F6
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionPhosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203