GTX60860 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.
![ICC/IF analysis of A431 cells nontreated or treated with Calyculin A/Okadaic Acid using GTX60860 Phosphothreonine antibody [RM102]. Red : Primary antibody Blue : DAPI Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX60860/GTX60860_20200909_ICCIF_190_w_23061123_646.webp "ICC/IF analysis of A431 cells nontreated or treated with Calyculin A/Okadaic Acid using GTX60860 Phosphothreonine antibody [RM102]. Red : Primary antibody Blue : DAPI Dilution : 1:500")
![IP analysis of Calyculin A/Okadaic Acid treated A431 cells using GTX60860 Phosphothreonine antibody [RM102]. Lane 1 : Whole lysate control Lane 2 : IP by rabbit IgG control Lane 3 : IP by Primary antibody Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX60860/GTX60860_20200909_IP_238_w_23061123_389.webp "IP analysis of Calyculin A/Okadaic Acid treated A431 cells using GTX60860 Phosphothreonine antibody [RM102]. Lane 1 : Whole lysate control Lane 2 : IP by rabbit IgG control Lane 3 : IP by Primary antibody Dilution : 1:500")
![WB analysis of serum-starved A431 cells non-treated or treated with Calyculin A/Okadaic Acid lysates using GTX60860 Phosphothreonine antibody [RM102]. Dilution : 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60860/GTX60860_20200909_WB_327_w_23061123_844.webp "WB analysis of serum-starved A431 cells non-treated or treated with Calyculin A/Okadaic Acid lysates using GTX60860 Phosphothreonine antibody [RM102]. Dilution : 1:2000")
GTX60860 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.
Phosphothreonine antibody [RM102]
GTX60860
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NamePhosphothreonine antibody [RM102] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:100 - 1:500. IP: 1:100 - 1:500. IHC: 1:100 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM102
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203