ELISA of Mouse anti-phosphotyrosine antibody (GTX22287). Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 microg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB
![Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9) (GTX22287). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 microg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody:Goat-a-Mouse IgG [H&L] secondary antibody diluted 1:2,500 for 30 at room temperature. Block: 5% BLOTTO overnight at 4oC.](https://www.genetex.com/upload/website/prouct_img/normal/GTX22287/GTX22287_20160330_WB_w_23060620_403.webp "Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9) (GTX22287). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 microg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody:Goat-a-Mouse IgG [H&L] secondary antibody diluted 1:2,500 for 30 at room temperature. Block: 5% BLOTTO overnight at 4oC.")
![ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22287 Phosphotyrosine antibody [13F9]. Coating : 0.1 microg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22287/GTX22287_20240423_ELISA_39_24042320_339.webp "ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22287 Phosphotyrosine antibody [13F9]. Coating : 0.1 microg Dilution : 1:1000")
![WB analysis of various samples using GTX22287 Phosphotyrosine antibody [13F9]. Lane 1 : Phosphorylated EGFR in A431 whole cell lysate Lane 2 : Unstimulated A431 whole cell lysate Loading : 10 microg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22287/GTX22287_20240423_WB_40_24042320_571.webp "WB analysis of various samples using GTX22287 Phosphotyrosine antibody [13F9]. Lane 1 : Phosphorylated EGFR in A431 whole cell lysate Lane 2 : Unstimulated A431 whole cell lysate Loading : 10 microg Dilution : 1:1000")
ELISA of Mouse anti-phosphotyrosine antibody (GTX22287). Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 microg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB
Phosphotyrosine antibody [13F9]
GTX22287
Overview
- SupplierGeneTex
- Product NamePhosphotyrosine antibody [13F9]
- Delivery Days Customer9
- Antibody SpecificityReactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:4000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID13F9
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionPhosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203