IP analysis of K562 cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours using GTX25592 Phosphotyrosine antibody [IG2]. IP antibody:GTX25592 IB antibody : c-abl antibody IP reaction : 2microg antibody / 500microg lysate
![Tyrosine phosphorylated c-Abl and Bcr-Abl was performed following immunoprecipitation of K562 cell lysates using a c-Abl antibody, from untreated cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. WB was performed using GTX25592 Phosphotyrosine antibody [IG2] at a dilution of 1:250.](https://www.genetex.com/upload/website/prouct_img/normal/GTX25592/GTX25592_1791_WB_w_23060722_899.webp "Tyrosine phosphorylated c-Abl and Bcr-Abl was performed following immunoprecipitation of K562 cell lysates using a c-Abl antibody, from untreated cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours. WB was performed using GTX25592 Phosphotyrosine antibody [IG2] at a dilution of 1:250.")
![ICC/IF analysis of HeLa cells using GTX25592 Phosphotyrosine antibody [IG2]. Green : Primary antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX25592/GTX25592_685_ICC-IF_w_23060722_501.webp "ICC/IF analysis of HeLa cells using GTX25592 Phosphotyrosine antibody [IG2]. Green : Primary antibody")
IP analysis of K562 cells (left lane) or cells treated with 10uM of the tyrosine kinase inhibitor Gleevec (right lane) for 24 hours using GTX25592 Phosphotyrosine antibody [IG2]. IP antibody:GTX25592 IB antibody : c-abl antibody IP reaction : 2microg antibody / 500microg lysate
Phosphotyrosine antibody [IG2]
GTX25592
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NamePhosphotyrosine antibody [IG2] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100 - 1:1000. ICC/IF: 1:50 -1:200. IP: 2 microg. ELISA: 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDIG2
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionProtein kinases like platelet-derived growth factor and epidermal growth factor have the ability to phosphorylate tyrosine residues of other proteins like GTPase activating protein. Protein kinases play a prominent role in the regulation of normal growth as well as tumor growth. A number of retroviruses encode proteins with tyrosine kinase activity and infection with such a virus is associated with a 10-20 fold increase in phosphotyrosine on the virally encoded protein as well as on other cellular proteins. This antibody, from clone 1G2, is capable of specifically binding to proteins which contain phosphorylated tyrosine residues, but will not react with any other phosphorylated amino acids, proteins or nuecleotides, and is excellent for the study of growth factor receptors, oncogenes, and signal transduction.
- ReactivityOther Species
- Storage Instruction2°C to 8°C
- UNSPSC12352203