Luminex analysis of Clone RM111s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. The GTX60918 reacts only to phosphorylated Tyrosine.
![ICC/IF analysis of A431 cells nontreated or treated with EGF using GTX60918 Phosphotyrosine antibody [RM111]. Red : Primary antibody Blue : DAPI Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX60918/GTX60918_20200909_ICCIF_191_w_23061202_699.webp "ICC/IF analysis of A431 cells nontreated or treated with EGF using GTX60918 Phosphotyrosine antibody [RM111]. Red : Primary antibody Blue : DAPI Dilution : 1:500")
![IP analysis of EGF-treated A431 cells using GTX60918 Phosphotyrosine antibody [RM111]. Lane 1 : Whole lysate control Lane 2 : IP by rabbit IgG control Lane 3 : IP by Primary antibody Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60918/GTX60918_20200909_IP_239_w_23061202_461.webp "IP analysis of EGF-treated A431 cells using GTX60918 Phosphotyrosine antibody [RM111]. Lane 1 : Whole lysate control Lane 2 : IP by rabbit IgG control Lane 3 : IP by Primary antibody Dilution : 1:1000")
![WB analysis of serum-starved A431 cells non-treated or treated with EGF lysates using GTX60918 Phosphotyrosine antibody [RM111]. Dilution : 1:5000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60918/GTX60918_20200909_WB_328_w_23061202_497.webp "WB analysis of serum-starved A431 cells non-treated or treated with EGF lysates using GTX60918 Phosphotyrosine antibody [RM111]. Dilution : 1:5000")
Luminex analysis of Clone RM111s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. The GTX60918 reacts only to phosphorylated Tyrosine.
Phosphotyrosine antibody [RM111]
GTX60918
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NamePhosphotyrosine antibody [RM111] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
- Application Supplier NoteWB: 1:1000 - 1:5000. ICC/IF: 1:200 - 1:500. IP: 1:200 - 1:1000. IHC: 1:200 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM111
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203