WB (peptide competition) analysis of Y1 Adrenocortical cell lysates treated with phosphatase (Lane 5) using GTX25815 PKA alpha + beta (phospho Thr197) antibody prior incubated with the non-phosphopeptide (Lane 2), a generic (pT) peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of Y1 Adrenocortical cell lysates treated with phosphatase (Lane 5) using GTX25815 PKA alpha + beta (phospho Thr197) antibody prior incubated with the non-phosphopeptide (Lane 2), a generic (pT) peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
PKA alpha + beta (phospho Thr197) antibody
GTX25815
Overview
- SupplierGeneTex
- Product NamePKA alpha + beta (phospho Thr197) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 0.1-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Scientific Descriptionc-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop. Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203